Abstract
Objective
To evaluate the effect of low-intensity pulsed ultrasound (LIPUS) on the haematological dynamics of an acute inflammatory process after an iatrogenic muscular lesion.
Design
Controlled laboratory study.
Setting
Research laboratory.
Participants
Eighteen male Wistar rats (weight 350 to 450
g) were submitted to surgical incision in the biceps femoris muscle (approximately 50%), and subsequently subdivided into control (n=9) and LIPUS (n=9) groups.
Intervention
Ultrasound (1.0MHz) was applied at the lesion site in the pulsed mode (2ms on, 8ms off) at 0.4W/cm2 for 3minutes at 1, 8 and 24hours post-surgery.
Main outcome measures
In these periods, blood was collected through venepuncture of the retro-orbital plexus and evaluated for different types of leukocytes and erythrocytes.
Results
LIPUS reduced the total leukocyte count at 1, 8 and 24hours post-surgery (1hour, control 9017.2±481×103/mm3 vs LIPUS 6189.8±450×103/mm3; 8hours, control 8078.2±501×103/mm3 vs LIPUS 5371.3±378×103/mm3; 24hours, control 8192.3±646×103/mm3 vs LIPUS 6059.1±503×103/mm3; P<0.001). The monocyte count was reduced at 8 and 24hours post-surgery (8hours, control 815.5±126×103/mm3 vs LIPUS 375.4±70×103/mm3; 24hours, control 875.3±124×103/mm3 vs LIPUS 564.7±56×103/mm3; P<0.001). The number of segmented neutrophils was only reduced at 1hour post-surgery (control 5033.1±397×103/mm3 vs LIPUS 3594.8±191×103/mm3; P=0.006), and the lymphocyte count was only reduced at 8hours post-surgery (control 4759.7±459×103/mm3 vs LIPUS 2584.1±356×103/mm3; P=0.003). Changes were not observed in the concentrations of young neutrophils, polibocytes and erythrocytes.
Conclusion
LIPUS reduced aspects of the inflammatory process following an acute incisional muscular lesion.
Citation
Haematological effect of pulsed ultrasound in acute muscular inflammation in rats
Luis Ulisses Signori, Silvio Teixeira da Costa, Adão Felix Saurin Neto, Rodrigo Machado Pizzolotto, Cristiane Beck, Graciele Sbruzzi, Antônio Marcos Vargas da Silva, Rodrigo Della Méa Plentz
Physiotherapy 1 June 2011 (volume 97 issue 2 Pages 163-169 DOI: 10.1016/j.physio.2010.06.004)